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Collection and Shipment of Specimens

All samples will be shipped/delivered and received at our Main receiving area in King Fahad Medical Research Center (KFMRC), Jeddah.

Specimen Requirements for Cytogenetics (Karyotyping/FISH):

• Amniotic fluid: 30 ml directly in 3 different 15 ml conical sterile plastic tube

• Blood: Adult: 3-5 ml, Pediatric 2ml minimum, Fetal blood: 2 ml minimum in Sodium Heparin tube. It is important that a first draw, spicule-rich sample be collected. Invert immediately upon completion of sample collection to prevent the formation of clots. The FISH analysis for uncultured sample only applicable for urgent cases. Usually referred orders will be treated as routine and will be accommodated according to the laboratory working hours

• Bone marrow (BM): 2 ml in sodium Heparin tube. (It is important that a first draw, spicule-rich specimen to be collected) • Chorionic villus sample (CVS): Collect 5–25 mg in 15 ml in sterile conical tube filled with sterile transport medium • Cord blood: 2 ml (minimum) in Sodium Heparin tube

• FFPE: Formalin-Fixed Paraffin-embedded tissue: FFPE Slice (3 slices 10 micrometer in thickness)/FFPE Slide or FFPE blocks in sterile conditions

• Lymph Node: 2–3 mm thick center slice or wedge of lymph node or other Lymphomatous tissue in sterile container containing sterile tissue culture media

• Product of conception (POC): Collect specimen into sterile plastic container filled with transport medium or sterile saline

• Solid tumor: Collect viable and non-necrotic tissue measuring 0.5 cm or 1-5 g in transport medium

• Unstimulated peripheral blood (Leukemic blood): 3-5 ml in Sodium Heparin tube

Specimen Requirements for Chromosomal Microarray Analysis:

• Blood: Adult: 3-5 ml, Pediatric 2ml minimum, Fetal blood: 2 ml minimum in Sodium Heparin tube. It is important that a first draw, spicule-rich sample be collected. Invert immediately upon completion of sample collection to prevent formation of clots

• Bone marrow (BM): 2 ml in sodium Heparin tube. (It is important that a first draw, spicule-rich specimen to be collected)

• FFPE: Formalin-Fixed Paraffin-embedded tissue: FFPE Slice (3 slices 10 micrometer in thickness)/FFPE Slide or FFPE blocks in sterile conditions

Specimen Requirements for Molecular Genetic Analysis:

• Blood: Adult: 3-5 ml, Pediatric 2ml minimum, Fetal blood: 2 ml minimum in EDTA tube. It is important that a first draw, spicule-rich sample be collected. Invert immediately upon completion of sample collection to prevent formation of clots

• Bone marrow (BM): 2 ml in EDTA tube. (It is important that a first draw, spicule-rich specimen to be collected)

• FFPE: Formalin-Fixed Paraffin-embedded tissue: FFPE Slice (3 slices 10 micrometer in thickness)/FFPE Slide or FFPE blocks in sterile conditions

• Fresh tissue in 15ml sterile tube filled with RNA later solution

• Saliva: Freshly collected saliva in Oragene container per kit’s specific instructions

Specimen Requirements for NGS:

• Blood: Next generation sequencing panels and clinical exome sequencing require 6-10 ml (adult), 5 ml (pediatric) minimum, SNP arrays require 1 ml minimum in EDTA tube. Blood samples not accepted for patients that have undergone an allogenic transplant (e.g. bone marrow or peripheral stem cell)

• Chemotherapy patients: DNA quality may be affected if the patient has received chemotherapy within the last 120 days. Clients will be contacted to provide additional specimen if DNA quality is insufficient

• DNA: 1 µg of DNA in TE (10mM Tris-Cl pH 8.0, 1mM EDTA); preferred 100 µl at ~50 ng/µl concentration for multi-gene/NGS panels. DNA OD 260/280 ratio (preferred 1.7-1.9). Please send agarose picture with high molecular weight genomic DNA, if available

• Saliva: Freshly collected saliva in Oragene container per kit’s specific instructions

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